WebDec 11, 2024 · FITC reacts with a primary amine on the protein to form a covalent amide bond. FITC-labelled protein substrates/peptides, antibodies, peptide hormones are used as specific probes in enzyme kinetics, immunocytochemistry as well as flow cytometry and identification of receptors on target cells, respectively. WebPopular answers (1) While both fluorochromes are excited by the blue 488 nm laser their respective emission peaks (525 nm for FiTC, around 620 nm for PI) are far enough apart that you'd expect...
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WebDr. Kanal gained valuable insight into global health issues and health disparities from her journey to Vietnam, Uganda, and Kenya as part of an opportunity in international mission … WebFor example, if using a positive FITC-stained sample to apply compensation to the PE channel, the median of the negative and the FITC-positive populations should be equal in the PE channel. All channels into which … can i have an mri if i have screws
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WebJan 10, 2024 · FITC (was one of the first dyes which was used for fluorescence microscopy and served as a precursor for other fluorescent dyes like Alexa Fluor®488. Its … Web1. For flow cytometry: a 96-well v-bottom plate or FACS tubes for staining and running samples, as appropriate for your flow cytometer; capability of exciting at 488 nm and detecting emission at 525 nm (FITC) and 650 nm (propidium iodide). 2. For fluorescence microscopy: cell culture plates (or chamber slides) WebThe flow cytometry (FCM) application of the antibody products is important for the proteomics research. Sino Biological, Inc. has the completely validated antibody products, which are especially for the flow cytometry (FCM) application to human targets. The targets would be updated periodly. fitz and floyd winter white holiday